Journal: The FASEB Journal

Article Title: Expression of the LRRC52 g subunit (g2) may provide Ca 2+ ‐independent activation of BK currents in mouse inner hair cells

doi: 10.1096/fj.201900701rr

Figure Lengend Snippet: Figure 4. Immunolabeling of LRRC52 using a custom-made antibody in testis. A, B) Immunolabeling for LRRC52 in a 10-mm cryosection of testis from a 3-mo-old male FVB6F1 mouse using a custom-made rabbit anti-mouse LRRC52 antibody. A) The cross section through a seminiferous tubule shows punctate LRRC52 immunoreactivity in spermatocytes and spermatogonia (arrowheads) and in adjacent Leydig cells (stars). B) Magnification of an LRRC52-labeled cluster of Leydig cells (stars). The Cy3-fluorescence of the secondary antibody is displayed in cyan. Nuclei were stained in blue with DAPI. Scale bars: 50 mm (A), 15 mm (B).

Article Snippet: The following secondary antibodies were used: donkey anti-rabbit AF488 (ab150073, 1:500; Abcam), goat anti-rabbit Cy3 (111-166-046, 1:1500; Jackson ImmunoResearch Laboratories, West Grove, PA, USA), donkey anti-mouse AF488 (A21202, 1:500; Thermo Fisher Scientific, Waltham, MA, USA), donkey anti-rabbit Cy3 (711-166-152, 1:1500; Jackson ImmunoResearch Laboratories).

Techniques: Immunolabeling, Labeling, Staining